Cryopyrinopathy

H&E staining for infiltrating leukocytes into the skin of myeloid expressed NLRP3A350V mice developing Muckle-Wells Syndrome (a form of Cryopyrinopathy) (left) and NLRP3A350V mice expressing POP1 in monocytes and macrophages.

ASC specks

Immunofluorescence microscopy of the NLRP3 inflammasome in human macrophages after treatment with bacterial RNA, showing ASC (green), DNA (blue) and actin (red).

LPS-induced peritonitis

Myeloperoxidase (MPO) activity of neutrophils infiltrating the peritoneal cavity in response to i.p. LPS injection in wild type and POP1 transgenic mice.

POP1 prevents IL-1b release

IL-1b release was determined in response to NLRP3 inflammasome activation by ATP in LPS-primed wild type, ASC-/-, NLRP3-/- or POP1 transgenic macrophages.

POP1-based therapy

Myeloperoxidase activity of neutrophils infiltrating the peritoneal cavity in response to i.p. LPS injection in wild type mice administered either vehicle control or a cell permeable, POP1-based drug before LPS injection.

POP3-AIM2 co-localization

Immunofluorescence microscopy of POP3 and AIM2 in human macrophages after infection with Modified Vaccinia Ankara (MVA) virus.

POP1 expression

Transgenic POP1 expression in distinct subpopulations of CD115+ peripheral blood monocytes determined by multicolor flow cytometry in CD68-POP1 transgenic mice.

POP3 IHC

Immunohistochemistry of POP3 (brown) in inflamed human lung tissue, showing expression in CD68+ macrophages (red).

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Caspase-1 FLICA

Caspase-1 activity assay (FLICA) quantified by flow cytometry in BMDM of wild type and POP1 transgenic mice in response to activation of NLRP3 with LPS/ATP.

POP3 modeling

Homology modeling of POP3 is based on the structure of the AIM2 PYD. Shown is a ribbon diagram and electrostatic surface representation on a scale of -4 kT/e (red) to 4 kT/e (blue). The right panel is rotated by 180° along the vertical axis.

POP3-IFI16 co-localization

Immunofluorescence microscopy of the NLRP3 inflammasome in human macrophages after treatment with bacterial RNA, showing ASC (green), DNA (blue) and actin (red).